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BIOINFORMATICS ((:warning:DNA extraction
From any organisms
Verify…
BIOINFORMATICS 
Application of computational tools on molecular data including the means to acquire, analyze, or visualize such data
Molecular data = sequence of DNA, cDNA or protein
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Pattern recognition
Particular sequence has been seen before and particular characteristic can be associated with it
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Nucleotide BLAST:arrow_forward: Protein BLAST
(translated nucleotide > protein)
Protein BLAST:arrow_forward: Nucleotide BLAST
(protein > translated nucleotide)
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:warning:DNA extraction
- From any organisms
- Verify the DNA through AGE
- Agarose percentage α1/DNA size
:warning:PCR
- Amplify targeted DNA sequence
- Verify amplified DNA fragments through AGE
:warning:DNA sequencing
- Verify the amplified DNA fragments from PCR through DNA sequencing
- DNA sequencing- result output in the format of chromatogram
:warning:Bioinformatics
- Interpret message in the DNA sequence
- Usage of BlAST program provided by NCBI
- Result shown as BLAST output
- Abbreviation for Basic Local Alignment Search Tools
- A tool for searching gene or protein sequence databases for related genes of interest
- Principle: computer comparisons using Algorithm
- Find best matching pair INPUT :arrow_right: OUTPUT
- DNA sequence obtained from DNA sequencing results must be saved in FAST A format
- Nucleotide or amino acids are represented as single letter codes
- Description line always start with '>'
- Word following '>' is sequence ID without space between them
- No space between nucleotide or amino acids
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