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Protein (Steps of Protein Sample Preparation (Cell disruption (Variables,…
Protein
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Sample / loading buffer
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SDS: Denature all proteins by breaking down secondary structure of protein and make them negatively charge.
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Protein Quantification
A total amount of protein or concentration of protein in a sample can be determined by protein assay.
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SDS-PAGE
Apparatus
Electrodes, lid, gel box, casting stand, clamping frame and electrode assembly, class plates, plastic comb, casting stray and glass plate sandwich.
Principals
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The intrinsinc charges of protein are obscured by placing a strongly anionic (-ve charge) detergent, SDS, in both the sample buffer and gel running buffer.
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