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Topic 5: Recombinant DNA Technology (Restriction endonuclease, Agarose gel…
Topic 5: Recombinant DNA Technology
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Cloning vs gene cloning
Cloning: Reproduction of organism as a whole without sexual fertilization between male and female gametes
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Restriction endonuclease
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The enzyme show specificity towards specific sequence on DNA; as restriction site and cut at this site
Some of this create DNA fragments with single stranded overhanging end ( sticky ends or cohesive ends
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DNA ligase
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