Please enable JavaScript.
Coggle requires JavaScript to display documents.
PROTEIN (protein sample preparation (cell disruption (use chemical…
PROTEIN
-
protein function
Manipulation of DNA and RNA through processes
- DNA replication
- DNA recombination
- RNA splicing/editing
-
Act a catalyst
- act as maintain metabolic processes
-
-
quantification
A total amount of protein or concentration of protein in a sample can be determined by protein assay.
-
-
-
-
- Cells need to be disrupted (to break open).
- Cells can be disrupted using either mechanical or non-mechanical cell disruption method.
- Cell lysis and protein solubilization are ideally carried out in the sample/loading buffer
sample/loading buffer
-
-
SDS is necessary to denature all proteins by breaking down secondary structure of proteins and make them negatively charged
-
-