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METHODS IN GENE CLONING (Steps involving in Polymerase Chain Reaction…
METHODS IN GENE CLONING
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3) Insertion
The gene of intrest is inserted into the cut plasmid. DNA ligase is used to bind the sticky ends together. Thus two pieces of DNA are joined together. Recombinant DNA is formed.
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5) Screening
- The screening process is used to identify the transformed bacteria which have sucessfully taken up the recombinant DNA.
- Blue-white screening : Bacteria are placed in a culture medium containing antibiotic (penicillin) and x-gal.
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