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TECHNICAL SUMMARY (Objectives (Isolate antibiotic compounds, Elucidate…
TECHNICAL SUMMARY
Objectives
Isolate antibiotic compounds
Elucidate chemical structures of these compounds
Validate lead targets
Identify biosynthetic gene clusters
Methods
Isolating antibiotic compounds
Bacterial cultures
Extraction of small mol antimicrobals in organic solvents
Chemical structure of the compounds
Natural product isolation
Structure elucidation
Liquid chromatography
Plate screens
Mass spec
NMR + other analytical techniques
Validate lead targets
Test activity against clinically relevant bacterial strains
Category 2 conditions
Disk diffusion assays
Identify biosynthetic gene clusters
Genome sequencing
Bioinformatic analysis
Reasons
Antibiotic resistance
Wellcome trust priority
New targets/pharmaceutical companys
Need academic research
Funding scaled back in big pharma
Access to sponges
New repository of bacteria
Bacteria from extreme niches
Outcomes
new insights into the microbial consortia associated with deep-sea sponges;
proof-of-principle testing of natural products with antibiotic activity
establishing the Bristol Sponge Collection as an exciting new resource for drug discovery
Background
Importance/Impact