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Ideas for manuscript (NMJ Label (Definitive, Needs confocal and a red a…
Ideas for manuscript
NMJ Label
Definitive
Needs confocal and a red a-BTX
Check how
May need to bleach and remove skin
Culture
Viability
Confocal
MAP2 stain
Rhodamine
Cut nerve
Dissection
Difficult to see
Physical removal after back fill should block uptake
Anterograde
Load to nerve, if connection exists should fill muscle
Aldehyde fixative to label NMJs or gross structure
MAP2
Qualitative
Relies on being able to visualise
Could support cutting experiments
Cytoskeleton
Add inhibitor
Issue if the release requires the cytoskeleton
Add mutant
Mrs lot