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Fly Sleep (clkjrk (DD (Total sleep (Decrease (Hendricks 2003, MY DATA)),…
Fly Sleep
clkjrk
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tim, per, Pdp13, and vri RNA levels was low at both CT3 and CT15 in ClkJrk mutants
Larvae
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Collins 2012
DN1s
LNvS
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LNv expression of NaChBac, a bacterial voltage-gated Na+ channel that increases adult LNv excitability (Nitabach et al., 2006; Sheeba et al., 2008a) and larval LNv responses to light (Yuan et al., 2011), increased light avoidance scores
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hyperexciting LNvs in a per01background via NaChBac significantly increased levels of light avoidance, whereas
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LNvs most excitable when CLK/CYC activity is low andDN1s most excitable when CLK/CYC activity is high
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light avoidance levels increased significantly when DN1s were hyperpolarized with either dORKDC or mKir2.1 or ablated with Dti.
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low CLK/CYC activity decreases DN1 excitability and consequently increases light avoidance by reducing DN1-mediated inhibition
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TrpA1 activation of DN1s did not affect light avoid-
ance at CT12, we conclude that DN1s are already active at CT12. However, because DN1 activation reduces light avoid- ance at CT24, we conclude that DN1s are usually inactive at CT24.
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Around dawn, low CLK/CYC activity increases LNv excitability and reduces DN1 activity. With DN1s releasing minimal gluta- mate, the LNvs respond strongly to ACh from the visual system and promote the dawn peak in light avoidance. Around dusk, high CLK/CYC activity reduces LNv excitability but increases DN1 activity, causing glutamate release and inhibition of the response of the LNvs to ACh via GluCl, reducing light avoidance.
wild-type larvae display circadian oscillations in avoiding 150 lux light.This rhythm peaks at subjective dawn (CT24, where CT = circadian time, time in constant darkness) and is lowest at dusk (CT12)
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Per01
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WT
tim, per, Pdp13, and vri expression was high at both CT3 and CT15
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Cryb
tim, per, Pdp13, and vri RNA were high CT3 and CT15
WT
tim, per, Pdp13, and vri RNA levels were
low at CT3 and high at CT15 in control flies