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tissue culture (growth of cells and tissues separate from the organism,…
tissue culture
growth of cells and tissues separate from the organism
facilitated by
same genotypes
cell culture hood(laminer flow hood or biosafety cabinet)
incubator(humid co2 incubator recommended)
waterbath
centrifuge
refrigerator and freezer
cell counter(countess automated cell counter or heamacytometer
liquid nitrogen freezer or cryostorage container
sterilizer(e.g autoclave)
selection of the plant tissue
sterilization
establishment of explant
medium requirements
multiplication of the explant
subdivision of callus
root formation
deflaskation