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Microfluidic (Advantages (Perfusion (Eliminates media stagnancy), Reduced…
Microfluidic
Advantages
Reduced reagent use
Perfusion
Eliminates media stagnancy
Increased cell to media volume
Reduces cell use
Can be coupled directly to analytical chemistry devices
Can be embedded with biosensors
Automation
Disadvantages
Requires new protocols or often considerable amendment of established protocols.
Bespoke applications offer versatility but preclude standardisation
PDMS has several issues
Oxygen, pH
Hydrophobic
Absorption
Often lack environmental control
Some macroscale techniques will not work.
External control systems are complex
Solutions
Standardised platforms at the expense of bespoke application
User-friendly control systems