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Microscopes (Transmission Electron microscope (Problems: (Higher energy…
Microscopes
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Cell Fractionation
In order for cell fractionation to occur sample tissue must be placed in a COLD, ISOTONIC, BUFFERED solution
Sample tissue is blended in a homogeniser (blender) to break down the cell membrane/wall and release the organelles
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Ultra centrifugation: filtrate placed into tube spun round at different speeds and each time supernatant (top of tube) is removed and spun again until all organelles have been separated out into sediments.
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