A 45-year-old Singaporean man presents to the GP with lethargy for the last few months. The GP did a full blood count which shows that the patient is anaemic

unexplained.

Microcytic

Iron deficiency

Thalassemia

Normocytic

Blood loss

Haemolysis

Chronic disease

Marrow infiltration

Macrocytic

Megaloblastic

Failure of production of RBC

Nutritional

Reduced BM erythroid cells

Ineffective RBC formation

Chronic inflammation

thalassaemia

renal disease

b12

iron

folate

aplastic anemia

marrow infiltration by leukaemia / neoplasm

GI bleed, ulcer, malignancy

memorrhagia

pregnancy

malabsorption (coeliac disease, atrophic gastritis)

malnutrition

GU bleed

pulmonary haemosiderosis

nail flattening, koilonychia (concave nails), sore tonges, angular stomatitis, dysphagia, gastritis

serum ferratin

B12

Folate

deficiency of gastric IF - pernicious, gastrectomy

AI - IgG against gastric parietal cells

blood count and film, BM aspirate, b12/folate levels, Auto antibodies, test for B12 absorption (radioactive labelled b12, shilling test)

risk of thrombosis, hyperhomocysteinaemia

absorptive - crohn's disease, stagnant loop syndrome, tropical sprue, fish tapeworm, congenital malabsoption

malabsorptive - coeliac disease, tropical sprue, small bower disease / resection

pregnancy, haemolytic anaemia, myeloproliferative disorders

drug induced - folate antagonists, metabolic inhibitors, NO (prolonged use)

hereditary orotic aciduria

inherited

disorders of RBC cell membrane

hereditary spherocytosis

hereditary elliptocytosis

RBC cell metabolism

G6PD deficiency

PK deficiency

oxidative stress trigger

fava beans, drugs, infections.

jaundive in neonate.

rbc cannot generate ATP and become regid

Acquired

AI haemolytic anaemia

warm

cold

microangiopathic haemolytic anaemia

haemolytic uraemic syndrome

thrombotic thrombocytopenic purpura

Thalassemia

alpha

beta

Hb-barts - deletion of all 4 genes, death in utero

HbH - deletion of 3. splenomegaly, hepatomegaly, bone and growth changes, hypochromic microcytic RBC with poikolicytosis, polychromasia and target cells.

trait - deletion of 1. lower MCV, MCH.

excess a-chain - toxic. skull bossing an maxillary enlargement. hair on end radiological appearance of the skull.

electrophoresis

Sickle cell

complications - GU (loss of ability to concentrate urine), Skin (lower limb ulceration), Eyes (proliferative retinopathy, glaucoma), Hepatobiliary (liver damage, pigment gallstones)

sickle cell trait

no clinical problems, since there is enough HbA in red cells. 60%.

Anaemia of chronic disease

malignancy

RA

Connective tissue disorder

chronic infection

extensive trauma