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Industry in biothechnology (Human insulin production (2.Plasmids DNA from…
Industry in biothechnology
Applications of industrial biotechnology
Medicine
Agriculture: Transgenic plant production.
Soybean, corn, and cotton
Example
Penicillin from Penicillium fungi
Fermentation: Food and beverages production
Example
Dairy products (cheese)
Meat products (sausages)
Vegetable products (pickles)
Importance of biothechnology in industry
Create new products
Reduce costs
Protecting the enviroment
Steps of fermentation
2.Preparation & sterilization of the fermentation medium
3.Preparation of pure cell culture for inoculation of the
medium in the fermentation vessel
1.Sterilization of fermentation vessel & associates
4.Cell growth & synthesis of the desired product under a
specific set of conditions
5.Product extraction & purification
importance of fermentation products
(using enzymes) and their uses
Pectinase to degrades pectin to soluble components of fruit juice
α-amylase are used in the production of high fructose corn
syrup from corn starch
Lipase to enhances flavor in cheese making
Single-cell protein (SCP)
protein extracted from cultured
microorganisms
Example
Bacteria: Rhodopseudomonas capsulata
Fungi: Aspergillus oryzae
Yeasts: Saccharomyces cerevisiae
Example of the biomass used in
SCP production
Coconut waste
Banana pulp
Sugarcane bagasse
Chymosin production
3.Chymosin gene inserted into plasmid
4.Plasmid put into yeast cell
2.Copy of chmosin gene
5.Chymosin from modified yeast cell produced
1.Extracted sample of calf cells
Cellulosic Bioethanol Process
1.Pre treatment
2.Enzyme hydrolysis
3.Fermentation
4.Distillation
Human insulin production
2.Plasmids DNA from bacteria extracted
3.Plasmid DNA cut with restriction enzyme
1.DNA from human pancreatic cells extracted
4.Combined the DNA from pancreatic cells with bacteria plasmid to form DNA recombinant
5.Took DNA recombinant into the vector
6.Undergoing growth and produce mutilpying human insulin
7.Extraction and purification of human insulin
8.Human insulin produced