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Cell-Synthetic Surface Interaction (Cell Adhesion (Role (Tissue &…
Cell-Synthetic Surface Interaction
Cell Adhesion
Impt Phenomenon
Biological Processes
Platelet Deposition at vascular injury sites
Neutrophils & Monocytes recruitment @ inflammatory/infectious sites
Role
Tissue & Organ Formation
Generate traction for cell migration
Determine biocompatibility of synthetic implant materials
Types
Animal
Initial: Cells attach to surface by pseudopodial extensions
Over Time: Cell spread and form focal contacts
Microbial
Initial: Cells exhibit Brownian Motion as they are deposited near surface
Adhesion reversible
Over Time: Synthesis of polymers to attach cell to surface
Adhesion irreversible
Approaches
Gibbs Energies of solid/bacterium, S/L, Bacteria/Liquid interfaces
Colloidal Stability Theory
Physical Nature
Factors
Interactions
Specific
Receptor-ligand bond formation
Non-Specific
No bond formation
Electrostatic forces
Steric Stabilization
VDW Forces
Temp
Medium Conc
Small Ion Conc
Surrounding Medium pH
Protein Adsorption
Proteins
Properties
High MW
Heterogeneous Molecules
Differing polarity, charge and hydrophilicity regions
Amphoteric & Amphiphilic
Structure
Specific AA sequence
Secondary
Tertiary
Aqueous Enviro
Driving Force
Thermodynamic
Molecules condense onto surface
Result
Invoke conformational changes in protein
Denature protein
Vroman Effect
Compositional change of protein layer absorbed from plasma
Whole blood & plasma
In vivo
Non-Static Event
Can undergo changes with time