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test 4 (cloning vectors (cut dna sample (electrophoresis (arranges by…
test 4
cloning vectors
cut dna sample
electrophoresis
arranges by size
transform bacteria
select bacteria with desired plasmid
white ones have inserted DNA
blue ones don't have inserted gene
transposons
genetic variation
SNP
small repeats
DIP
deletion or insertion
SSR
one base pair change
CNV
blocks of duplication or deletion
genetic drift
founder effect
bottleneck effect
natural selection
population genetics
gene pool
monomorpic
no change in allele frequency
fixed
polymorphic
changes in allele frequency possible
evolution
change in frequency of alleles
Hardy Weinberg Law
large population size
no population movement
no mutations
random mating
no natural selection
genetic drift
mutations
allelic heterogeneity
locus heterogeneity
knockout
knockin
conditional knockout
not inactivated until conditions are met
pharming
transgenic animals to make protein drugs
gene targeting
heritability
quantitative
associative mapping
DNA libraries
cDNA
comes from mRNA
genomic editing
CRISPR/Cas9