Please enable JavaScript.
Coggle requires JavaScript to display documents.
Microscopy (Electron microscopy (Beam of electrons with wavelength less…
Microscopy
Seeing is believing
-
-
-
-
Cell theory:
Both plant and animal tissue is composed of cells
Cells are the basic unit of all life
Cells only develop from existing cells
Light microscopy
-
-
-
-
Observe dead, prepared specimens
-
-
-
-
-
Magnification, resolution, and the magnification formula
-
-
-
-
-
-
Electron microscopy
-
-
Magnifications up to x500,000
-
-
-
-
Transmission electron microscope (TEM): beam electrons transmitted through specimen/ focused produce image
Resolution: 0.5nm
Scanning electron microscope (SEM): beam of electrons sent across surface specimen and reflected electrons collected
Resolution: 3-10nm
3D images of surface
-
-
-
Creation of artefacts
-
-
Preparation specimens for electron microscopy changes in ultrastructure cells inevitable (loss in continuity membranes, distortion organelles, empty spaces in cytoplasm of cells)
-
