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AZM (Resistance (Mutation in rpIV and rpID (Also confers resistance for…

- - - - Specific response to AZM binding Ribosome
      - Mutation = increase unprocessed 23s-5s rRNA
  - - - Possibly
        
        Modify 23s rRNA
        
        OR increase intracell Aminoacyl-tRNA via Recycling peptidyl-tRNA
- - - - Rather, sub-regulon (small fraction of QS-dependent PROTEOME)
        
        Perhaps Rare Codon more affected? (especially when its first few AA prior due to "Drop-off" effect resulting in tRNA depletion)
        
        107 transcripts & 43 proteins
      - Consider multi-layered study? Yichen did transcriptome already, how about proteome to factor in post-transcriptional and translation regulation?
- - - - Ribosome Drop-Off
        
        rhIR transcript
      - Premature peptide chain termination
      - Block Enlognation
      - Ribosome Stalling
        
        Depletion of free tRNA
        
        Differential effects due to codon and tRNA biases in genome
        
        Nascent peptide chains of covalently bonded tRNA
    - - DIRECT: Stall and Drop-Off
        
        Likely due to Ribosome binding
      - INDIRECT: tRNA pool 'rescue' especially skewed for certain important sub-regulons?
      - Effect on rescuing sRNAs? E.g. Against mRNA binding proteins
        
        MsrE does not bind nucleic acid though
        
        Unlikely?
    - - Structural Approach
        
        Crystallography
        
        SER
        
        Original
        
        Proteolysis
        
        CryoEM
        
        +/- tRNA & mRNA
        
        Generic vs Targeted
        
        Specific mRNAs/ tRNA/ peptidyl-tRNA
  - - - Layered with Tecan/ Transcriptome/ Proteome/ mRNA footprint reading?