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Optogenetics (VTA role in addiction receiving dopamine from the VTA (STh,…
Optogenetics
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Types of Nuerons
Neuron activators Channelrhodopsin (ChR2)
NA and Calcium are let in
Stimulate with Bluet Light
Lets positive ions into the cell
Opsin should be tagged with a fluorescent tagged so that you can visualise the infected neurones. GFP or Mcherry
Function should always be checked using electrophysiology
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Activation of CHR2 on oxytocin terminals in the CEL of the central amygdala reduces fear in rats. Tested using oxytocin receptor antagonist.
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