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Cytoplasm and Cytoskeleton (Cytosol (Contains 200-400mg/ml protein (2…
Cytoplasm and Cytoskeleton
Cytosol
70%Water where organelles and other molecules float.
Separated from external environment by plasma membrane which prevets free flow of molecules in or out
Sphere = smallest surface area to volume ratio
Contains 200-400mg/ml protein
(2 TYPES)
1) Filamentous protein structures: - remain after detergent.
CYTOSKELETON
Do not dissolve in cytosol and give cell shape
2) Soluble globular proteins: - can be removed by detergents
Microtubules
Cytoskeleton
Protein fibres arranged into bundles or 3D networks , filaments are held in bundles and networks by
CROSS LINKING PROTEINS
Functions
Determine intracellular location of organelles
Specialised functions
Chromosome separation
Locomotion (motile cells)
Establish cell shape and cell mechanical strength
Cytoskeleton filaments = long polymers of single monomeric proteins
3 filament types
Intermediate Filaments
Largest
- Microtubules
Smallest
- Actin filaments (microfilaments)