iBOL- The International Barcode Of Life project

Who are they?

How does it work?

What species have been identified?

Recent news/current projects

DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species.

system of species identification and discovery using a short section of DNA from a standardized region of the genome. That DNA sequence can be used to identify different species, in the same way a supermarket scanner uses the familiar black stripes of the UPC barcode to identify your purchases.

The gene region that is being used for almost all animal groups, a 648 base-pair region in the mitochondrial cytochrome c oxidase 1 gene ("CO1")

proving highly effective in identifying birds, butterflies, fish, flies and many other animal groups

The advantage of using COI is that it is short enough to be sequenced quickly and cheaply yet long enough to identify variations among species.

The COI barcode is not effective for identifying plants because it evolves too slowly, but two gene regions in the chloroplast, matK and rbcL, have been approved as the barcode regions for land plants.

How does it happen? #

Species identification using DNA barcodes starts with the specimen. Barcoding projects obtain specimens from a variety of sources. Some are collected in the field, others come from the vast collections housed in natural history museums, zoos, botanical gardens and seed banks

In the laboratory, technicians use a tiny piece of tissue from the specimen to extract its DNA

The barcode region is isolated, replicated using PCR amplification and then sequenced

The sequence is represented by CATG

Once the barcode sequence has been obtained, it is placed in the Barcode of Life Data Systems (BOLD) database - a reference library of DNA barcodes that can be used to assign identities to unknown specimens.

Nodes

Countries that join are known as nodes

What nodes do

Lobby for funding for DNA barcoding

Obtain funds for barcoding facilities

Identify national priorities

Nations involved

Central- support the facilities to share information with all nations involved

Europe

Finland

Germany

Netherlands

Portugal

Britain

France

United States

China

Canada

National- Focus on collecting and identifying specimens from their own countries

Colombia

Costa Rica

Kenya

Korea

Pakistan

Madagascar

Panama

Papua New Guinea

Peru

Regional- maintain core sequencing facilities

Argentina

Australia

Brazil

India

Mexico

New Zealand

Norway

Russia

Saudi Arabia

South Africa

Campaigns

Formicidae Barcode of Life

All Birds Barcoding Initiative

12,000 Ant species

Collected DNA barcodes from five or more individuals of all of the approximately 10,000 bird species

Trichoptera Barcode of Life

A long-term project to barcode the world's approximately 13,000 species of caddisflies

Fish Barcode of Life Initiative

Campaign to barcode all the species of fish

Lepidoptera Barcode of Life

Aims to barcode all the species of butterfly and moth, with separate regional campaigns in Australia and the US

The Mammal Barcode of Life

Part of a larger effort aiming to encompass all vertebrates, this one just aims to barcode all mammals

Mosquito Barcoding Initiative

The MBI plans to barcode at least five specimens from 80% of the 3,200 known mosquito species. Disease-bearing species and their closest relatives are the priority.

Marine Barcode of Life

Aims to barcode the worlds oceans

Polar Barcode of Life campaign

Co-ordinates efforts in Antarctica and the Arctic, on both land and sea