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Central Dogma (DNA (Trascription (Translation (Protein), (Uses promoters…
Central Dogma
DNA
Trascription
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Nucleotide specific is Uracil,
Uses promoters
Same process as DNA Intiation, elongation, termination
G-Cap: adds to RNA after it is made to protect it, ticket for RNA to leave nucleus
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PolyA tail: stabilizes the RNA, it gives the mRNA enough time to code for proteins before it is degraded
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Intron: intervening sequences not coding for a protein as it is being made into mRNA it is being cut and spliced out as its being read
Exons: get to stay, they code for peptides, will be translated and in final stuff.
miRNA: micro RNA made by cells, is complementary to a portion of messenger RNA; end result. no protein ribosome blocked
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Nucleotides make up DNA, T is specific
CIs element : a DNA Sequence, upstream from gene
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Genome
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cDNA Libray: take messenger RNA, Specific to cell type and tissue type
Epegentics
Methylation
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CG islands methylation on cis element prevents transcription factor from coming in and can prevent gene expression or enhance gene expression, depends on the amount of methylation