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BIOCHEMICAL TEST (SULFIDE, …
BIOCHEMICAL TEST
INDOLE TEST
To determine if the organism can produce the enzyme tryptophanase which cleaves tryptophan, generation indole
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SULFIDE, INDOLE, MOTILITY (SIM) TEST
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Sulfur in the media may be reduced to hydrogen sulfide(H2S) ; this appears as a blackening within the media
If indole is produced, a 'cherry-red' band forms on the surface of the media
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CATALASE TEST
To determine if the organism produce enzyme catalase that breakdown hydrogen peroxide generating oxygen and water
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MOTILITY DEEPS
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If you see a "fanning" of growth , or lack of a stab line, then it is motile
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OXIDASE TEST
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- Use sterile swab to pick up a colony and add a few drops of oxidase reagent to swab
- Moist filter paper with the substrate (1% tetramethyl-p-phenylenediamine dihydrochloride)
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UREASE TEST
To determine if the organism can produce the enzyme urease, which cleaves urea generating ammonia
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XLD TEST
Selective and differential agar (selects for gram negative rods, also helps differentiate Salmonella from Shigella
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