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Feb 2016 (Housekeeping Gene: High (Reconstitute Primers (Validate in…
Feb 2016
Housekeeping Gene: High
Reconstitute Primers
Validate in control RNA
Test New Genes using xray-induced Senescence RNA
Get 1 more biological donor RNA for senescence
Present workflow diagram at progress
53BP1/H2AX staining
Flow Cytometry: High
CD45-e450 Titration
Scale-up Test (10 million events)
Plan AutoMACS experiment with CD14/CD235a beads
Repeat Glycophorin A staining
Cord Blood Studies: Medium
Genotype - 2 donor (cord + PB)
CD14
CD206
CD163
Immunophenotype
CD163
CD206
CD14
Matrigel (Much later)
Other Projects: Low
Drug Studies
Repeat
Image Analysis
Joana's Retinas