Please enable JavaScript.

Coggle requires JavaScript to display documents.

Smears & Stains (Simple Stains (enables us to visualize the shape and…

- - - - The Question: What kind of cell wall does this organism have?
      - The Procedure:
        
        1) Primary Stain - Crystal Violet
        
        2) Apply a mordant - Iodine
        
        3) Decolorize - w/ Alcohol or Acetone-alcohol, Differential Step
        
        4) Counterstain - Safranin (Pink)
      - The Results:
        
        G+: Violet
        
        G-: Pink
    - - The Question: Does this organism have the waxy cell wall of mycobacterium?
      - The Procedure:
        
        1) Primary Stain - Carbol Fuchsia
        
        2) Heat - forcing stain inside the waxy cell wall
        
        3) Decolorize - Acid Alcohol
        
        4) CounterStain - Methylene Blue
      - The Results:
        
        Acid-Fast: Fuchsia colored
        
        Non-Acid-Fast: Blue