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Microscopy (Light Microscopy (Applications (Brightfield: fixed (dead),…
Microscopy
Light Microscopy
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Applications
Brightfield: fixed (dead), stained specimens (for contrast)
Darkfield: living, unstained, morphology (outside shape)
Phase Contrast: living, unstained, morphology and internal detail
Immersion Oil: The light may bend in air so much that it misses the small high magnification lens. Immersion oil is used to keep light from bending (or being lost)
General information
Magnification = ocular lens (10) x objective lens (10,20,40)
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