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QGEM Thermocycler (Programming and interior parts (Interface (Buttons or…
QGEM Thermocycler
Programming and interior parts
Interface
Buttons or direct input from computer
Screen to display time, temperature and cycles
Arduino/IDE
Look for code in online sources
How to prevent overheating Arduino Chip
Solid Edge
Analyse properties of materials
Designing interior parts
Matlab code for efficiency
Cooling Source
Different ways of cooling
Water cooling
Fan
Cooling coils
water vs air
refrigerator unit
Rate of cooling
Time Constraints
Heat absorption/dissipation of materials
Safety Regulations
Sensor
Layout
"Control" Tube Setup
Multiple Sensors
Sensor Type
Submersible
Thermocouples
Thermistors
RTD Probes
sensors in the thermocycler
sample block temp sensor
coolant temp sensor
heated lid temp sensor
ambient temp sensor
Power Source
Battery powered or wall outlet
Is portability important
How much power does the thermocycler need
How will it be programmed
Best sensor to use
Arduino
Heating System
Wrap coils around PCR tubes
Add metal inserts/sleeves for even heat distribution
Potentially difficult to implement
Heated Bath
Require powerful heating source
Potentially inefficient
Materials
Research different materials to construct thermocycler
Body
Heated Lid
Price of Materials
Heat capacity
Outer shell/container
Workshops to build in
3-D Printing
Wood
holding lock of PCR tubes
Insulation for safety purposes
PCR tube holder
Plastic
Heated sheath
Metal sheet w/ holes