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Antigen-Antibody reactions (Agglutination (Hemagglutination use RBC as a…
Antigen-Antibody reactions
Agglutination
Direct Agglutination
Slide Agglutination
Tube Agglutination
Indirect/Passive Agglutination
Indirect Hemagglutination test (IHA)
RBCs are used as carrier molecules
Latex Agglutination Test
for antibody detection
polystyrene latex particles (1um) are used as carrier molecules
:female-scientist::skin-tone-4: antistreptolysin O antibody (ASO)
Reverse Passive Agglutination
for antigen detection
Reverse passive hemagglutination assay
Latex agglutination test
for antigen detection
Coaggulation test
Hemagglutination
use RBC as a source of antigen
Paul Bunnell test
sheep RBC , Epstein barr virus
Cold agglutination test
mycoplasma antibodies, Human rbc
Blood grouping (ABO and Rh)
Coombs test
direct and indirect
Complement Fixation test
Wasserman test - syphillis
other tests
antibodies capable of binding to complements are detected
this technique is almost obsolete now
Neutralization test
less commonly used