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Unknown Sample (Gram Negative (TSIA (Red slant/Yellow Butt - ferments…
Unknown Sample
Gram Positive
Coagulase
Clumping - (+) in coagulation
No clumping - (-)
MSA
Poor Growth - (-) Staphylococcus
Good Growth - (+) Staphylococcus
Yellow growth/Halo - Possibly S. aureus
Red growth/No halo - Other Staphylococcus
Catalase
Bubbles (+)
No change (-)
SFM
Color Change: Yellow/Brown - (+), fermentation of dextrose
E. faecalis
E. faecium
No change
Spores
NSA
Growth - (+)
Endospore staining
Poor Growth - (-)
Bile Esculin
Darkened within 48hrs
Group D Streptococcus or Enterococcus
No change after 48hrs
Not members of Group D Streptococcus or Enterococcus
Cell Morphology
Cocci
Bacillus
Gram Negative
Methyl Red
Red (+)
Yellow (-)
Voges-Proskauer
Red/pink (+)
No change (-)
Motility
Whole tube cloudy (+)
Cloudy around stab (-)
Citrate
Color change (+)
No change (-)
Indole
Red (+)
Yellow (-)
Urea
Orange --> pink (+)
No change (-)
H2S
Black (+)
No change (-)
EMB
Poor growth - G(+)
Good Growth - G(-)
Dark with metallic sheen - possible coliform
Colorless - Noncoliform
Pink and mucoid - possible coliform
TSIA
Red slant/Yellow Butt - ferments glucose only
Yellow slant/Yellow Butt ; Ferments all 3 sugars
No change - no sugars fermented
MacConkey
Poor growth - G(+)
Good growth - G(-)
Pink/red w/o ppt - possible coliform
Colorless - noncoliform