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Complete blood count (CBC) (RBC (Calculated parameters (Mean corpuscular…
Complete blood count (CBC)
WBC
White cell count
นับ cell ที่มี nucleus ทุกตัว
Corrected WBC count
Series count
Neutrophils
Absolute #
ยึดค่านี้เป็นหลัก
Neutrophils (%) x corrected WBC / 100
Percent %
Lymphocytes
Monocytes
Eosinophils
Basophils
White cell count (x10^3/ul) x 100 / (NRBC (%) + 100)
Leukopenia : low
Leukocytosis : high
Absolute NRBC #
Platelet
Platelet count
Ref value
150-450 x 10^3/ul
Thrombocytopenia : low
Thrombocytosis : high
RBC
Measured parameters
Red cell count, Hemoglobin, Hematocrit
High : Polycythemia
Polycythemia vera
Genetic
Stay high altitude
Congenital heart disease
Low : Anemia
Aplastic anemia
Pancytopenia
Chronic renal failure
ไตสร้าง erythropoietin
Iron def.
Pregnancy
Dilutional effect
WHO Criteria
Adult male
Hb < 13 g/dl
Adult female and child 6-12 yrs
Hb < 12 g/dl
Pregnancy and child < 6 yrs
Hb < 11 g/dl
Rule of three
Apples to
normocytic, normochromic RBCs only
Useful to
detect lab error
Red cell count —x3–> Hemoglobin —x3–> Hematocrit
Calculated parameters
Mean corpuscular volume (MCV)
Average volume (size) of RBC
Ref value
80-100 fL
Hct (%) x 10 / RBC x (10^12/L)
Normochromic anemia : 80-100 fL
Acute blood loss
Hemolytic anemia
Macrocytic anemia : >100 fL
Folate def.
B12 def.
Microcytic anemia : <80 fL
Thalassemia
Iron def.
Mean corpuscular hemoglobin concentration (MCHC)
Conc. of Hb in volume of total RBCs
Hb (g/dL) x 100 / Hct (%)
Ref value
33-37 g/dL
Spherocytosis (Hyperchromic)
Hereditary spherocytosis
Hemolysis
Hypochromic
Thalassemia
Iron def.
Red cell distribution width (RDW)
Variation in RBC size
Bell curve graph
SD of MCV x 100 / Mean MCV
Ref value
11.0-14.5 %
Anisocytosis
B12/Folate def.
Iron def.
Hemolytic anemia
RBC fragmentation
Properties
ISO 15189
Specimen
EDTA blood
รักษาสภาพ cell โดยดึง Ca2+ ออกจาก cell
Should be performed within 4 hrs
Unless
Lymphocytes
Clover leaf nuclei
RBC
False positive echinocytes
Techniques
Electrical impedance (EI)
ใช้กระแสไฟฟ้า
Cell detection by
electrical resisitance
Amplitude of each pulse associate cell size
Light scttering (LS)
ใช้ลำแสง
Cell detection by light scatter
Forward
cell volume or size
Side
internal complexity (content/granules) บอกชนิดของ WBC