Please enable JavaScript.
Coggle requires JavaScript to display documents.
PHYTOCHEMISTRY (Phytochemical Plant Analysis (ISOLATION & SEPARATION…
PHYTOCHEMISTRY
Phytochemical Plant Analysis
ISOLATION & SEPARATION
Column Chromatography
GC
HPLC
Combination
PHYTOCHEMICAL SCREENING TEST
Quantitative ; check amount of phytoconstituents
Qualitative ; check present/absence of phytoconstituents
IDENTIFICATION
Qualitative ; IR, NMR
Quantitative ; UV, MS
EXTRACTION
Choice of solvents
Effect of extracted plant phytochemicals
Extraction methods
TYPES OF EXTRACTION
SOLID-LIQUID extraction
SOLVENT extraction
DECOCTION
woody crude ; with heat
INFUSION
soft phytoconstituents ; not heat
PERCOLATION
HOT
COLD
CONTINUOUS HOT (SOXHLET EXTRACTOR)
DIGESTION
heat plant first b4 mix w solvent ; for thermostable subs (not for volatile subs)
MACERATION
HOT
COLD
COUNTERCURRENT extraction
Screw extractor
Carousel extractor
SUPERCRITICAL GAS extraction
STEAM DISTILLATION
LIQUID-LIQUID extraction
Separating Funnel
ANALYSIS OF RESULTS
Qualitative ; PC, TLC, HPTLC
Quantitative ; GLC, HPLC, MS
Constituents
Active Constituents ; responsible for pharmacological actions
Inert Constituents ; not responsible pharmacological actions
Microbiological conversions
Amikacin synthesis from Kanamycin by aminoglycoside producing strain of Bacillus circulars through addition of hydroxy amino butyric acid