chapter 19, viruses

structure of viruses

they consist of nucleic acid that enclosed by protein coats, for some have a membranous envelope

viruses can have double-stranded DNA, single-stranded DNA, double and single stranded RNA depending on the type of virus.

a DNA virus or an RNA virus based on the nucleic acid that makes up the genome

capsids and envelopes

capsids are the protein shell that enclose the viral genome

capsids are built from a large number of protein subunits called capsomeres

tobacco mosaic virus, rod-shaped capsid made from over thousands of molecules a single protein in a helix shape

adeno viruses, infect animals respiratory tract, proteins arranged polyhedral capsid that has 20 triangular facets called an icosahedron

there are some viruses that have structures that allow them to infect their hosts

influenza virus has a membranous envelope that surrounds the capsid

bacteriophage (phages), complex capsids found in viruses that infect bacteria

the capsids have icosahedral heads that enclose the DNA, attached to the head is a protein tail piece that have fibers in which the phages attach to a bacterial cell

viruses replicate only in host cells

viruses don't have metabolic enzymes and equipment in order to make proteins such as ribosomes

but viruses can only replicate within a host cell, b/c they are intracellular parasites

their is a limitation to what a virus can infect when it is in a host called the host range

viruses recognize the host cell by a "lock-and-key", allowing it to fit between viral surface proteins and specific receptors molecules on the outside of the cell

cycle of phages

the lytic cycle, lead up to the death of the host cell

it refers to the last stage of infection, the bacterium lyses and the phages are released that were produced

the phages infect the healthy cells, and the lytic cycle can destroy bacterial populations in just a few hours

a virulent phage is a page that replicates only by the lytic cycle

the lysogenic cycle, allows the replication of the phage genome w/ destroying the host

there are phages that can use both modes of replication with a bacterium are called temperate phages

replicative cycles of animal viruses

animal viruses have an envelope called the outer membrane allowing it to enter the host cell

on the outside of the envelope are viral glycoproteins that bind to a certain receptor on the host cell

cycle with the enveloped virus with a RNA genome

(1) ribosomes bound to (ER) of host cells make are able to make the protein parts of the envelope glycoprotein.

(2) the (ER) and the Golgi apparatus have cellular enzymes which add sugars

(3) resulting viral glycoproteins. embedded in the membrane derived from the host cell are transported tot eh cells surface

(4) new viral capsids are wrapped in membrane as they bud from the cell

(5) the enveloped viruses are free to infect other cells

the replicative cycle don't kill the host cell

RNA as viral genetic material

three types of single stranded RNA genomes in animal viruses

genome class of IV viruses serve as mRNA and are translated into viral protein after infection

evolution of viruses

an isolated virus in biologically inert, so it cant replicate its gene or make its own ATP

even though viruses cant replicate or carry out metabolic activities independently

viruses have been found that infect every form of life (bacteria, plants ,fungi, animals, archaea etc.) b/c they depend on cell for reproduction

evolution of genes coding for capsid proteins have allowed viruses to bind cell membranes, facilitating infection to uninjured sites

viral diseases in animals

viruses can damage or kill cells from the release of hydrolytic enzymes

viruses cause the infected cells to produce toxins, producing symptoms.

the damage that viruses cause depends on the ability of the infected tissue to regenerate

temporary symptoms of viral infections is a way the body defends itself against infection

vaccine, harmless variant of the pathogen that stimulate the immune system to mount defenses against the harmful pathogen

b/c antibiotics kill bacteria by inhibiting enzymes specific to bacteria, and have no effect on viral encoded enzymes

ex: smallpox a viral infection

emerging viruses

suddenly become apparent

ex: HIV and AIDS, Ebola, encephalitis

epidemic, wide outbreak in Mexico and United States known as influenza virus (H1N1)

pandemic, global epidemic

viruses burst on to the human scene in three ways

first is the mutation of existing viruses b/c RNA have a high mutation rate, but do not proofread and correct errors in replicating

second, mergence of viral is the spreading of viral disease in small isolated populations

some mutations change existing viruses into new genetic varieties

their use of genetic codes makes it hard to deny evolutionary connection

ex: AIDS went unnoticed until it began to spread

third, viral diseases in humans spread of existing viruses from other animals

animals that carry a virus are unaffected by it, and are considered natural reservoir for the virus

ex: swine flu, from pig to human

three types of influenza virus

type B, and C infect only humans never caused an epidemic

type A, infect a wide range of animals, birds, horses, pigs and humans

different strains of A are given names

name identifies tell us which form of two viral proteins are present, hemagglutinin (H), and neuraminidase (N)

(H) there are 16 different types, a protein that helps the flu virus attach to the host

(N) there are 9 types, enzyme that helps release new virus particles from infected cells

viral diseases in plants

common sights of vial infection in plants

bleached or brown spots on leaves or fruits, stunted growth, damaged flower or roots

viral diseases spread either horizontal or vertical tranmisson

horizontal a plant is infected by from external source of the virus, it has to get past the protective layer

vertical, inheriting viruses from parent

a plant becomes more vulnerable to viral infection if it was damaged by wind, injury or herbivores

herbivores are a threat b/c the act as carriers of viruses

occur in asexual propagation or in sexual reproduction

virus enter plant cell and replicates genomes and proteins it can spread throughout the plant by means of plasmodesmata

plasmodesmata, the cytoplasmic connections that penetrate the walls between adjacent pant cells

passage of viral macromolecules cell to cell, is facilitated by virally encode proteins that enlarge the plasmodesmata

viroids and prions

viriods circular RNA molecules that infect plants

they don't encode proteins but use host cell enzymes

viriods cause errors in the regularity hat control plant growth

typical signs are abnormal development and stunted growth

prions an infectious protein

causing degenerative brain diseases in animal species

ex: mad cow disease, scrapie in sheep, Creutzfeldt-Jakob disease in humans

prions act slowly with an incubation period of about ten years before symptoms develop

secondly prions are indestructible

prions are misfolded versions of normal brain proteins

the prions convert the normal proteins to the misfolded prion

prion aggregation interferes with normal cellular functions and cuases disease symptoms

lysogenic signifies prophages capable of generating active phages that lyse their own host cells

a switch over from lysogenic to lytic cycle is triggered by chemical or high energy radiation

class V, the RNA genome serves instead as a template for mRNA synthesis

the RNA genome is transcribed into RNA strands, functioning as mRNA and as a template for synthesis of additional copies of genomic RNA

viruses that use RNA genome as template for mRNA transcription require RNA to RNA synthesis

most complicated RNA animals viruses is the retrovirus

virus equipped with an enzyme called reverse transcription

transcribes the RNA template into DNA, RNA to DNA information flow the opposite of the usual direction

ex: HIV that causes AIDS