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Relative Masses (Mass Spectrometer (Ionisation (Electrospray Ionisation,…
Relative Masses
Mass Spectrometer
Electrospray Ionisation
The sample is dissolved and pushed through a small nozzle at a high pressure. A high voltage is applied to it, causing each particle to gain a H+ ion. The sample is turned into a gas made up of positive ions.
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The positively charged ions are accelerated by an electric field so that they all have the same kinetic energy. This means that the heavier ions move slower than the lighter ones.
The ions enter a region with no electric field, so they just drift through it. Lighter ions will drift faster than heavier ions.
Because lighter ions travel at a faster speed, they reach the detector in a faster time. The detector detects a charged particle and a mass spectrum is produced.
Mass Spectrum
Y-axis
The abundance of ions, often given as a percentage.
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