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Biology

Bacterial Growth

Serial dilutions

Aseptic techniques

Microscopy

Cells

Characteristics of life

However, in order to be able count individual colonies the concentration of bacteria in the sample must be low enough so that you do not end up with the agar plate completely merged together (a lawn). Very often you will need to dilute the original sample before growing them on the agar plate. A serial dilution is a method that is frequently employed to produce a set of solutions of decreasing concentration.

A single bacterium is far too small to be seen by the naked eye, and even under the school microscopes they will look like a small dot at best. Therefore you can't just put a sample of the bacteria under the microscope and count the number of bacteria present.

Additional features of all organisms

MRS GREN + Homeostasis

Genetic material (nucleic acids)

DNA is an example of a nucleic acid. The other type of nucleic acid found in organisms is RNA (ribonucleic acid).

Viruses, although not living organisms, also contain a nucleic acid - some have DNA whereas others have RNA. When a virus infects a cell, it injects its DNA or RNA into the cell, and the infected cell then uses it to make more copies of the virus proteins.

DNA is the molecule which provides instructions for how a cell is built and functions. In simple terms, the DNA molecule is a molecular code which determines which proteins are synthesised by the cell. The precise type of DNA that a cell contains determines which organism the cell belongs to; in other words, the DNA in the nucleus of a frog cell is different from the DNA in the nucleus of a human cell.

All living organisms contain DNA (deoxyribonucleic acid) in their cells. In Prokaryotes (bacteria), the DNA is a single loop located in the cytoplasm. In Eukaryotes, the DNA is packaged using proteins into multiple linear strands called chromosomes.

Growth

Reproduction

Sensitivity

Excretion

Respiration

Nutrition

Movement

Homeostasis

Nonetheless, we do consider movement one of the characteristics of life since all cells are able to move components around inside the cytoplasm, even if this is not immediately visible to the naked eye.

Some plants have the ability to move tissues or organs, such as the opening and closing of stomata, and Venus fly traps can rapidly close their traps. Both of these are reversible, and so considered movement. The way in which plants 'bend' towards the light, however, is in fact a growth response and irreversible, and so not considered movement.

Many animals have the ability to move in the sense of locomotion (moving the whole organism from one place to place), although of course plants and some animals such as corals are fixed in one place.

Respiration is the release of energy (usually I the firm of the molecule called ATP (Adenosine Triphosphate)) through the oxidation of an energy source (such as glucose).

Note that this kind of 'cellular' respiration is not respiration in the sense of breathing (taking air into the lungs), although the oxygen we breathe is used in cellular respiration. In prokaryotes like bacteria, respiration takes place in the cytoplasm but in eukaryotes respiration takes place in specialised organelles called mitochondria.

Aerobic respiration requires oxygen; anaerobic respiration can occur in the absence of oxygen and is carried out by some bacteria, some fungi and to a very limited extent by human muscles. This is similar to the process by which lactobacillus produces lactic acid used in the process of making yoghurt.

Sensitivity is the ability to respond to changes in their surroundings. In large multicellular organisms this is either through nervous systems or by using hormones (chemical messengers carried in the bloodstream). In plants, responses usually take the form of changes in the pattern of growth, and are regulated by chemicals.

In unicellular organisms, sensitivity is usually confined to moving in response to stimuli such as light or to chemicals.

Organisms can grow by either enlarging cells by increasing the volume of cytoplasm by cell division, which will increase the number of cells in the organism.

Multicellular organisms carry out both kinds of growth whereas when single-celled organisms divide the two individual daughter cells are considered different organisms.

Reproduction is a feature of all organisms. Most organisms reproduce sexually which leads to offspring that are variable. Some organisms may reproduce asexually, in which case the offspring are genetically identical to each other and to the parent offspring.

Excretion is the removal of waste products from an organism. The key part of this definition is that the substances excrete were produced by the organism. Examples include getting rid of carbon dioxide (a waste product from aerobic respiration), and urea (from the breakdown of excess proteins). Excretory products need to be removed from the cell or from the organism as they are often toxic at high concentrations.

Note that excretion is different from egestion. Egestion is the removal from the body of undigested food in the form of faeces. The cellulose cell walls of plants is a large component of faeces; humans are unable to digest cellulose and so this passes through the digestive system unchanged.

Nutrition is the obtaining of chemicals for either use in respiration to release energy or for use in the building of new molecules with which to construct new cells.

A cell or an organism may be described as carrying out heterotrophic or autotrophic nutrition. Heterotrophic organisms use pre-existing food molecules which they may digest and use. Animals are examples of heterotrophs. Autotrophic organisms synthesise food molecules from simpler molecules. All photosynthetic organisms are autotrophic - they use carbon dioxide and water to synthesise simple sugars such as glucose.

The most common nutrients you will encounter are glucose (a simple sugar) that is the main substance used in respiration and amino acids which are used to synthesise proteins.

Homeostasis is the maintenance of constant internal conditions despite changes in the external environment.

For complex multicellular organisms such as humans, this includes many factors such as keeping the concentration of blood (i.e. water balance), temperature, blood pH and the concentrations of mineral ions within strict limits

For unicellular organisms such as bacteria, they clearly have less of an ability to control factors such as temperature, but they still regulate the chemical composition of the cytoplasm such as pH, water levels and mineral ion concentrations.

Plants carry out respiration just like animals (and therefore use up oxygen) as well as carrying out photosynthesis (which produces oxygen)

Microscope components

Units

Scale and magnification

Sometimes there may be a scale bar given instead - the actual length of the bar in the image (e.g. measured in millimeters) represents the stated length (usually in micrometers) of the real object.

Magnification is how many times larger a microscope makes an object look. The number of times an object has been magnified is often showed on or beside a picture so you can work out its actual size.

Diaphram

Light source

Focus knob

Stage

Eyepiece lens

Objective lens

Works in combination with the eyepiece to magnify the image; there may be several to chose from on a rotating turret. The school microscopes have a 4x, 10x and 40x lens.

Tis is the one you look down; the school microscopes have a 10x one.

Where the specimen (slide) goes. There are usually clips of some description to hold it in place.

There are various types, but all vary the amount of light passing through the specimen. The diaphragm is just below the stage.

Sometimes a bulb, sometimes just a mirror.

One for fine and one for coarse focus.; you should always focus with both hands to avoid straining the mechanism.

Most common units

1m ÷ 1000 = 1mm 1mm ÷ 1000 = 1μm

millimeters (mm)

micrometers (μm)

meters (m)

Wash your hands.

Sterilise the work surface before you start.

Light a bunson burner near where you are working to stop anything in the air landing on your bacteria.

Cell theory

All cells have

Cell structure

Two major types of cells

Eukaryotic

Prokaryotic

Eukaryotic cells make up all organisms other than bacteria - i.e. plants, animal, fungi and the protoctista. They contain an extensive set of membrane-bound compartments, notably the nucleus, mitochondria and in photosynthetic cells chloroplasts. Structures in a cell that carry out a particular function such as chloroplasts, mitochondria and ribosomes are known as organelles.

They are only found in bacteria and contain fewer components. In particular, prokaryote lack a membrane-bound nucleus, in which the DNA is packed into chromosomes. Prokaryotic cells tend to be much smaller than eukaryotic cells with a few exceptions.

Cytoplasm

Genetic material in the form of DNA

A cell surface membrane (also known as a plasma membrane)

Ribosomes

  1. The cell is the smallest unit of life.
  1. Cells can only be derived from other cells.
  1. All organisms are made of cells.

Mitochondrion

Cell wall

Ribosomes

Chloroplast

Nucleus

Vacuole

Cytoplasm

Bacteria only

Cell surface membrane

Capsule

Flagellum

Plasmids

A small, extra loop of DNA. Plasmids may contain extra genes and they are used extensively in genetic engineering to transfer new genes into bacteria. Bacteria may have one or several plasmids.

Not always present, this is a layer of protective slime surrounding the cell wall.

Not always present, this allows bacteria to move. In bacteria it acts as a kind of motor, rotating the long flagellum and propelling it through water.

Plant and fungal cells contain a large permanent vacuole. This is a membrane-bound compartment that is used for storage of molecules such as dissolved sugars, mineral ions, pigments (e.g. in petals). The watery contents of the vacuole is known as cell sap. Although similar structures can be found in animal cells (we refer to these as vesicles), they are small and temporary.

Cells of the green parts of plants have chloroplasts. Like mitochondria, chloroplasts are enclosed by a membrane that separates their contents from the rest of the cytoplasm. Chloroplasts absorb light energy to make food in the form of sugars such as glucose in the process of photosynthesis. Chloroplasts are green because they contain a green pigment called chlorophyll. Plant cells that aren't green such as root cells lack chloroplasts.

Since bacteria don't possess chloroplasts, all of the reactions of photosynthesis must take place in the cytoplasm.

Cell walls have large holes in them, so it is not a barrier to water or dissolved substances. It is freely permeable.

The cell wall is a layer of non-living material that is found outside the cell membrane of some cells. In plants, it is made of cellulose (a carbohydrate made from glucose), in fungal cells it is made from chitin and in bacteria it is made from peptidoglycan (a mixture of carbohydrates and amino acids).

The cell wall is a tough layer that helps the cell keep its shape and why it is plants have a fixed shape; animal cells, which lack a cell wall, can be more variable in shape. The contents of a plant cell (vacuole and cytoplasm) push out against the cell wall which give the plant support.

All living eukaryotic cells contain mitochondria. Cells which have a high demand for energy, such as muscle cells, have large numbers of mitochondria. Some of the reactions of respiration take place in mitochondria.

Since bacteria don't possess mitochondria, all of the reactions of respiration must take place in the cytoplasm.

All cells, Prokaryotes and eukaryotes, contain ribosomes. These are too small d be seen with a light microscope, but are present in the cytoplasm. Proteins are made on ribosomes.

The cytoplasm is a gelatinous material predominantly made up of water with many substances dissolved in it. Other structures described below such as the nucleus, ribosomes and mitochondria are found in the cytoplasm. Many chemical reactions of the cell happen in the cytoplasm.

This is a thin layer that forms a boundary between the cytoplasm and the outside. It is mostly made up of lipids and proteins. The basic structure of the cell surface membrane is the same in both prokaryotes and eukaryotes. Cell membranes are selectively permeable. This means that the cell is able to control what substances can enter or leave the cell.

Eukaryotic cells contain a membrane-bound nucleus. A few cells such as red blood cells or mature xylem cells (which lacks any cytoplasm and are dead ) lack a nucleus. The nucleus controls the activities of the cell. This is done through the DNA which determines which protein a cell can make.

In eukaryotes the DNA is packaged into several linear chromosomes. Human cells contain 46 chromosomes, although other species have different numbers of chromosomes. The DNA in chromosomes is arranged into sections called genes; one gene codes for one specific protein. Although chromosomes vary in length, they can contain several hundred to a few thousand genes each.

In labs they are grown on agar

Bacteria reproduce by splitting in two, a process called binary fission. Bacteria will form discrete colonies when grown on agar or cause cloudiness in a liquid.

Requirements

Oxygen if they respire aerobically

Mineral ions

Nutrients such as glucose

Water

A suitable temperature

Fungi

Identification keys

Evolutionary trees

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The five kingdoms

Classification

Protoctista

Plants

Animals

The Linnaean system

The Binomial system

This system organises organisms into smaller and smaller groups

Order

Family

Class

Genus

Phylum

Species

Kingdom

A species is defined as a group of organisms with many characteristics in common wich can interbreed and produce fertile offspring

In the binomial system an organism is referred to by its genus and species name together (e.g. Homo sapiens or Tyrannosaurus rex)

The correct convention for writing binomials

The genus is capitalised but the species is not

They should also be either italicised or underlined if handwritten

An identification key is a set of questions that are used to put a name to an organism.

Usually keys are made to identify a particular species, or to all species of a specific group.

Identification keys are also sometimes called dichotomous keys because the questions are grouped into two pairs (e.g. organism is radially symmetrical versus organism is bilaterally symmetrical)

An evolutionary tree or a phylogeny is a pictorial representation of how species are related to each other.

The intersection between two branches (where they meet) is called the node

phylogeny example 2

The node represents a common ancestor, now extinct

phylogeny example 1

Viruses, because they are not considered living organisms are not in any of the kingdoms

Organisms can be divided into two larger groups

All living organisms have been classified into five kingdoms

Prokaryotes

Eukaryotes

The plant kingdom contains the land plants. They are all multicellular, terrestrial organisms that

Have cellulose cell walls

Store carbohydrate in the form of starch.

Have chloroplasts and are able to carry out photosynthesis

There are four major groups of land plants

Pteridophytes

Gymnosperms

Bryophytes

Angiosperms

Mosses, liverworts and hornworts

About 20,000 species

Ferns and their relatives

About 13,000 species

Such as the conifers

About 1,000 species

Flowering plants

About 300,000 species

The three main characteristics that define the various groups of lands plants

Possession of seeds

Possession of flowers and fruits

Possession of vascular tissue

Specialised xylem and phloem which conduct water, mineral ions or sugars and amino acids throughout the plant

Seed plants reproduce using pollen rather than dispersing spores, and the embryo that forms is contained within a seed

Most plant species are flowering plants in which the seeds are held in the ovary, which becomes the fruit

The difference between land plants and algae

The defining character is that the young embryo plant is retained on one of the parent plants,whereas in algae both the male and female gametes are dispersed from the parent organism

The proper name for the land plants is Embryophytes which reflects this important character

plant phylogeny

These plants do not have phloem or xylem

These plants reproduce using spores

These plants use seeds which are held in cone like structures

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These plants reproduce using spores When the spores germinate, they produce motile male gametes (like very simple sperm) and so they are restricted to wet habitats

These plants do not produce seeds

These plants do not produce seeds

Algae are actually protoctista not plants, though they are the closest relatives

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General characteristics

Structure

Reproduction

Yeasts

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Structure of the body cavity

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Fungi are eukaryotic and heterotrophic

They have no chloroplasts and cannot photosynthesis

Fungi have cell walls made of chitin

Fungi feed by extracellular digestion. They secrete enzymes onto their food and absorb the smaller molecules that are released, such as glucose and amino acids

They are usually saprotrophic (digesting dead organisms or material) but some are parasitic and will ive in plants or animals

Fungi are some of the largest organisms in the world by mass

They grow a network or a mycelium of fine, branching, hair like structires called hypae, which can extend over many miles

Fungi periodical;ly send up fruiting bodies which produce reproductive spores

The spores can be produced sexually or asexually

Some of these fruiting bodies are very large and are called 'mushrooms' or 'toadstools'

Yeasts are single celled fungi and there are thousands of different types

They reproduce asexually by budding, in which a small cell grows out of the side of an existing one

They can also reproduce sexually using a special reproductive cell called a shmoo

Shmoo's only have half the normal number of chromosomes and fuses with another shmoo of the opposite sex to form a zygote

Examples

Plasmodium

Paramecium

Amoeba

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