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Fungi (Diagnosis (Cytology of exudate/tissues (Gram stain, Lactophenol…
Fungi
Diagnosis
Clinical behaviour and appearance of lesions
Cytology of exudate/tissues
Gram stain
Lactophenol cooton blue
Indian ink
KOH
Fungal culture and appearance
Histopathology (Biopsy or PM)
Periodic acid -schiff
Gram stain
Immunoflourescence
Molecular methods
PCR
Serology
Animal incoulation
Forms
Yeast
Types
Cryptococcus
Malassezia
Candida
Unicellular spherical cells
Reproduce asexually
Facultatively anaerobic
moulds (filamentous)
Types
Aspergillus
Microsporum
Trichophyton
Multicellular filaments
Septate or non septate, vegetative or aerial
Reproduce sexually (spores) or asexually (conidia)
Fluffy/cottony colonies (unlike bacteria)
Stricly aerobic
Dimorphic fungi (mould 25C and yeast at 37C)
Sporothrix schenckii
Histoplasma capsultum
Coccidioides immitis
Fungal Disease
Infectious (Parasitic Fungi) - Mycoses
4 Main types
Mucusal
Systemic /Deep fungal infections
Zyogomycetes
Pigs
gastric ulcers
submandibular lymph-node lesions
Catttle
Sporadic abortions
Fatal pneumonia
Dogs
GIT ulcers
Cryptococcus
Hosts
Cat/dog
CNS lesions
Blindness
Sub cut and nasal granulomatous
Horse
nasal granulomatous discharge
Cattle
Chronic mastitis
People
Lungs and CNS Meningitis
Pathogensis
Inhaled may spread through locally or disseminated
Cutaneous
Disease
Candidiasis (thrush, enteritis, mastitis)
Dermatophysis ( ring worm)
Microsporum
M.Canis
Morphology
3 more items...
M.Gypseum
M.Nanum
Trichophyton
T. Mentagrophytes
Morphology
3 more items...
T. Verrucosum
Asperigillus
Birds
Extremely common
targets
Air sac
pneumonia
GIT
Systemic
Horse
Guttural pouch mycosis
nasal granulomatous
Cattle
Pneumonia
Mycotic abortion
mastitis
Subcutaneous
Disease
Eumycotic mycetoma
Sporotrichosis