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SFI Frontiers 2020 (Apparatus (CB2Rfl/flGFP-CD4Cre mice, Humanised mouse…
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Alt Aim 1
Aim 1A
Using human PBMC, we will examine the impact of cannabinoid receptor activation on glycolyiss pathway and alterations in gut homing receptor expression.
Alternative Approach
Using Jurkat/EL4 cells we will examine the impact of cannabinoids on gut homing moluecule expression
Aim 1B
We will crisper/cas9 out CNR2 from human T cells and determine the ipamct on integrin expression, phenotyome and function in vtiro