In the lab
Need culture medium (liquid gel, for nutrients)
Provides MO w/ necessities; Carbs, energy
Various minerals, nitrogen (protein)
Warmth, Oxygen also needed
Hot agar gel (petri dish) poured, cools, add MOs
OR flask, sterile nutrient broth solution (alt.)
Use uncontaminated MO culture to investigate
Avoid contaminate - skin, air, soil, water
Mutation - possible new, dangerous pattern