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CI activity composition (Development (Mitochondrial function…
CI activity composition
Activity
Physiological assay
Find original reference
Could be supplemented by in gel assay but this is non-physiological
Normalisation?
Composition
Tied to activity
45 sub-units
Fingerprinting technique
Development
ATP demand
Mitochondrial function
Supercomplex
Fertilisation changes
Disease
Linked to multiple diseases
Check point
What does the literature say on CI in general in development
Xenopus
Unknown
Resource
Design
Simple
Positive control
Two time-points
Western blot
In gel digestion
Unresolved
Whole egg?
Contamination
Use BN PAGE?
Could be in mitochondria
When to do the Bradford
Themes
Development
Xenopus
Mitochondria
Complex I