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PROTEIN STRUCTURE and FUNCTION (THE SHAPE AND STRUCTURE OF PROTEINS…
PROTEIN STRUCTURE and FUNCTION
THE SHAPE AND STRUCTURE OF PROTEINS
Protein: main building blocks for cell
protein conformation(shapes) is determined by AA sequence
AAs are linked by
peptide bonds
Non-covalent bonds decide the final conformation
hydrogen bonds
electrostatic attractions
van der Waals attractions
hydrophobic interactions
i.e. hydrophobic side chain containing AAs tend to gather and facing inwards of the protein
chaperone proteins
help folding protein into conformation
Secondary Struc
alpha helix
e.g. alpha-keratin; skin, nails and hairs
result from H-bonds between N-H and C=O within the polypep backbone
coiled coil
several a-helices wrap around one another - facing non-polar inwards
beta sheet
probs;
amyloid fibres
insoluble protein aggregates which accumulates within the cell
e.g. Alzheimer's disease, prison disease
Primary struc
AA seq
たんぱく質の構造
アミノ酸２０種類
極性アミノ酸１０種類
非極性アミノ酸１０種類
必須アミノ酸 アラニンー リシン＋
HOW PROTEINS ARE CONTROLLED
regulation of protein activity
inhibitory proteins
binding site
HOW PROTEIN WORK
binding site
conformational change
働く仕組み
BCAA
HOW PROTEINS ARE STUDIED
renaturing
protein can be
denatured
but at the right conditions, proteins folds back to its right conformation once denaturing solvent is remmoved
showing that all the information needs to fold proteins into the right conformation is within AA sequences
mass spectro
chromatography