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E.anophelis carbapenemases related (20180909主任提供的想法 (這兩隻菌，到底差異在那，或許可用你最近常用…
E.anophelis
carbapenemases related
metallo-beta-lactamases(MBL)
GOB
BlaB
Extended-spectrum β-lactamase (ESBL)
CME
Methods and Materials
DNA level
已證實所有的EA菌皆帶有blab和GOB基因
已證實所有的EA菌皆帶有CME基因
mRNA level
目前遇到CME的部份無法夾出預期片段
phenotypic methods of MBL
A lawn culture of test isolate (0.5 McFarland opacity standard) was done on MH agar. Two 10 μg imipenem discs were placed on inoculated plates. To one of the imipenem discs, 10 μl of 0.5 M EDTA solution was added. After overnight incubation, if the zone of inhibition of imipenem + EDTA discs compared to imipenem alone is >7 mm, the test was considered as positive
combined disc diffusion test
Precious studies revealed cephalosproin resistance due to CME, where BlaB responsible for carbapenems(imipenem or meropenem)
20180909主任提供的想法
這兩隻菌，到底差異在那，或許可用你最近常用blast方式來比對。
1.針對GyrA mutation的菌，先作出有那段mutation 的完整序列。
2.再來跟whole sequence 此対，看看符合度到底多少。
3.再拿這段，跟另一whole sequence，看看符合度到底多少。
4.小心謹慎比対後，有可能發現差異處，就可以寫以NGS為主的文章。或是加在目前正在寫的quinolone 文章內。
另外確定G(-)幾乎都有acrAB pump。
2011AAC for
E.menigo