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Wrap-ups for Paper (Phagocin procution (Absolute Western Blot (Comparison…
Wrap-ups for Paper
Phagocin procution
Absolute Western Blot
Purified Standard protein Load
Troubleshooting:: Things different from Denish's Band
Phagocin Loss during processing
Protease Digestion during DNAse Step
sensitive to centrifugation
sensitive in high concentration
Lack of chloroform streatment
Wrong band from CsCl
separate and take all bands (isopycnic gradient)
comparative western blot for major capsid protein abundance
Find the right band and corresponding density
paralell SYPRO ruby gel for protein concentrations
Comparison of mutant production
Comparison of starting OD?
TCA prep
Comparison of ability to concentrate?
normal lysate prep
KFU Assay
says 10^11-10^13 meaning a burst size of around 100
Fluorescent Microscopy
TEM-- tail lengths and TEM PTA contractions
Tail Fiber Switching