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Production (Purification from natural, De novo chemical synth,…

- - - - Planar chromatography
      - column chromatography (larger volume or separation distance)
      - immobilized
        metal ion affinity chromatography (IMAC)
        
        subtype : Histag ; imidazole: competing agent
      - Adsorption chromatography
        
        retention of the absorbateby the absorbent = polarity
        
        Eluent flow in the mobile phase
      - High Pressure Liquid Chromatography (HPLC)
  - - - Migration
        through a pH gradient
      - migrate to their respective
        pIs
    - - pH gradient first then rotated field and migrate through SDS gel