Please enable JavaScript.
Coggle requires JavaScript to display documents.
CHAPTER 9 CHROMOTOGRAPHY = SEPERATION (2 phases (stationary phase, mobile…
CHAPTER 9
CHROMOTOGRAPHY =
SEPERATION
2 phases
stationary phase
mobile phase
property =
immiscible
:!:
supercritical fluid :question:
NO equilibrium state
prior to chromatography: countercurrent extraction = ancient apparatus
PRINCIPLES
Plot
: (the peaks of) concentration in stationary phase vs. distance along column
distribution constant
retention time
typical for specific analytes
sometimes need to include a detector to define (the nature of) peaks
TYPES
adsorption
not often used
stationary phase = solid
Partition
stationary phase =
liquid
reversed phase
is more commonly used