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Mycobacterium tuberculosis (Lab (Sputum examination (2 samples 8 hours…

- - - - Lymph node
      - Brain
      - Pleura
      - Kidney
- - - - Talking
      - Sneezing
      - Coughing
  - - - Tuberculin positive
      - ทำลายเชื้อได้ ไม่ก่อโรค
    - - Primary lesions 5%
        
        Granulomatous tubercle at cervical lymph node
    - - May remain latently decades
      - Secondary lesions
        
        Caseous necrosis
        
        Calcification
        
        Cavitation
- - - - At least 5 ml
    - - Spot พบแพทย์
      - Morning
  - - - Kinyoun (cold stain)
        
        Requires 5,000-10,000 AFB per ml of sputum
      - Options
        
        Carbol- fuchsin 5 min > 3% acid-alcohol 2 min > methylene blue 30 sec
      - ย้อมจาก Culture
        
        Serpentine cord
  - - - Digestion
        
        N-acetyl-L-cysteine
      - Decontamination
        
        NaOH
    - - Ogawa (solid)
        
        Soldium glutamate
        
        Rough, dry, buff, nonpigment (สีเหลืองฟางข้าว, ดอกกะหล่ำ)
  - - - Positive
        
        Fluorescence
        
        TB - aerobic bact. ใช้ O2
      - Ruthenium
        
        Sensitive ต่อ O2
    - - ใช้ตรวจ TB complex
      - Protein Ag ที่ specific กับ cell wall
- - - - Resistant to isoniazid-rifampicin
    - - Resisitant to isoniazid-rifampicin-fluoroquinolones